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(2000) in yeast mutants exhibiting either a UGA suppressor phenotype (mutations I35F, V71I, L126V and H131R) or a UAG suppressor phenotype (mutations M51I, S77F and D113G). Residues shaded in gray were supposed to be involved in stop codon [url=http://www.ttcarpets.co.uk]mulberry outlet[/url] recognition: dark gray (Liang et al., 2001; Muramatsu et al., 2001), light gray (Knight and Landweber, 2000; Lehman, 2001; Lozupone et al., 2001). I35V, M51L and L126F (Lozupone et al., 2001), and L126I in Euplotes, where UGA encodes cysteine (Figure 1).
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In keeping with the proteomic data (Figure 1), annexin 2 was readily detected (Figure 2A, main panel) by western blotting in complexes precipitated using an anti S100A6 antibody ( from Panc 1 cell lysate. For reference, the detectable levels of annexin 2 in Panc [url=http://www.wiis.fr]louboutin[/url] 1 whole cell lysate are shown, as are the levels of S100A6 in the precipitated and whole cell lysate samples (Figure 2A, lower panel). Reciprocal immunoprecipitation with an annexin 2 antibody ( followed by western analysis with an anti S100A6 antibody revealed the specific detection of S100A6 in complexes precipitated from Panc 1 cell lysate (Figure 2B, main panel).
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